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Growth characteristics of fibroblasts isolated from neck and limb of Caspian miniature horse: In vitro study

fibroblasts of caspian

According to the results of this study, there is a noticeable deference in growth behavior of neck fibroblasts in comparison with the limb fibroblasts in Caspian miniature horse .
Omid Azari1, Ladan Emadi 2 , Hojjat Gholipour 3

۱Department of Clinical Sciences, 2 Department of Basic Sciences and 3 Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman

۱۶th National and 4th International Conference of Biology, Ferdowsi University of Mashhad, Mashhad, Iran , ۱۴-۱۶ September 2010

Summary

Exuberant granulation tissue is the most common complication of equine limb wounds, which consist primarily of developing fibroblasts and their protein products that form the surrounding matrix.

The in vitro studies showed that growth rate in fibroblasts isolated from the horse limb is less than the fibroblasts isolated from the horse trunk and pony limb; but there is no difference in cell growth characteristics between limb and body fibroblasts in ponies .

According to extensive researches on genetics and phenotype of Caspian miniature horses, it is strongly confirmed that Caspian horse is a horse, not a pony.

In this study 3 grams of subcutaneous tissue was harvested from the neck and metacarpi of four Caspian miniature horses and placed on culture medium separately .

After separation of fibroblasts from the harvested tissues, using explant culture method, a concentration of 10000 cells/ml was placed into each well of a 24 -well plate, and then cell counting was carried out after 4 days .

The cell growth rate and viability percentage were compared between neck and limb fibroblasts.Results of this study showed that the mean fibroblast growth rate from the neck of Caspian horses was significantly greater than that for the limb )P<0.05 .(There was no significant difference
in viability percentage between the neck and limb fibroblast (P>0.05 ).

According to the results of this study, there is a noticeable deference in growth behavior of neck fibroblasts in comparison with the limb fibroblasts in Caspian miniature horse .

Keywords: Caspian miniature horse, Fibroblast, Limb, Neck

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