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Growth characteristics of fibroblasts isolated from the body and limb of the Caspian miniature horse and the effect of hydrocortisone in vitro

Hydrocortisone

The present study was designed to determine the in vitro effects of hydrocortisone on the growth of fibroblasts isolated from both the neck and distal limb of the Caspian miniature horse.

O. Azari (*) : M. Karamouzian
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran , e-mail: omidazari@mail.uk.ac.ir
L. Emadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman,
Kerman, Iran
S. M. Ghamsari : M. M. Dehghan
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Received: 3 February 2010 /Accepted: 25 August 2010 / Published online: 4 September 2010
# Springer-Verlag London Limited 2010

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Abstract

The present study was designed to determine the in vitro effects of hydrocortisone on the growth of fibroblasts isolated from both the neck and distal limb of the Caspian miniature horse.

Three grammes of subcutaneous tissue was harvested from the neck and metacarpi of four Caspian miniature horses and tissue from each site was placed in a separate culture medium.

After separation of fibroblasts using explant culture method, a concentration of 10,000 cells/ml was placed into each well of a 24-well plate. Hydrocortisone was added in three different concentrations
(control group: without hydrocortisone, treatment groups A, 10; B, 100 and C, 300 μg/ml) into the culture medium containing fibroblasts, 4 days later the cells were counted.

The effects of hydrocortisone on fibroblasts growth and viability were evaluated. Results show that the
mean fibroblast growth rate from neck-derived fibroblasts was significantly greater than those derived from the limb.

There were no significant differences in fibroblast growth rate between the control group and group A in both neck and limb-derived fibroblast (P>0.05), but groups B and C suppressed fibroblast growth significantly in comparison with their related control groups (P<0.05). Cell viability decreased significantly in treatment groups B and C in comparison with the control group in both neck and limbderived fibroblasts.

The mean percentage of fibroblast growth inhibition from the neck and limb significantly decreased in treatment groups B and C in comparison with their related control groups. The effect of hydrocortisone on cultured fibroblast growth was dose-dependant.

Keywords: Hydrocortisone , Fibroblast culture, Neck, Limb , Caspian miniature horse

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