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Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses

sperm quality , کیفیت اسپرم

This study aimed to determine the effects of different centrifuging protocols (different rotation and time) on Caspian horse sperm quality. Hoosahng Noori1; Armin Towhidi2; Majid Bahreyni3; Tooba Nadri4
۱Former M.Sc. Student, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
۲Associate Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
۳Veterinary Expert, Animal Breeding Center of Iran, Karaj, Iran
۴Ph.D. Candidate, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Article 1, Volume 47, Issue 4, Winter 2017, Page 481-487 XML PDF (581 K)
DOI: 10.22059/IJAS.2017.138918.653396

ِAbstract

This study aimed to determine the effects of different centrifuging protocols (different rotation and time) on Caspian horse sperm characteristics.

In this study, semen samples from four healthy adult Caspian pony stalian were collected using an artificial vagina and then diluted in milk phosphocaseinat based extender.

Then semen samples were divided into seven equal parts, which part assigned to treatments including centrifugation treatments (500×g, 1000×g and 2000×g each for 4 and 7 minutes) and without centrifuge (control).

Cooling of treatments were done at 0, 24 and 48 hours after centrifugation. Sperm motion characteristics, viability and percentages of healthy sperm were assessed.

The results showed that motility, viability and percentages of healthy sperm reduced in 2000×g treatments (p<0.05). Stored sperm in 500×g and 1000×g treatments had better quality compared to control group (p<0.05).

In addition, compared to 2000×g treatment, in 500×g and 1000×g treatments a less recovery rate was observed (p<0.05). In conclusion, Caspian horse’s semen in treatment with centrifuging for 7 minutes at 1000 g, showed better result.

Keywords:  Healthy , matility , quality evaluation , sperm , viability

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